home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9406d.zip
/
M9460666.TXT
< prev
next >
Wrap
Text File
|
1994-06-25
|
2KB
|
37 lines
Document 0666
DOCN M9460666
TI Identification of CD7 glycoprotein as an accessory molecule in
HIV-1-mediated syncytium formation and cellfree infection.
DT 9408
AU Sato AI; Balamuth FB; Ugen KE; Williams WV; Weiner DB; Department of
Pathology, University of Pennsylvania, Philadelphia; 19104.
SO J Immunol. 1994 May 15;152(10):5142-52. Unique Identifier : AIDSLINE
MED/94230996
AB A major cytopathic effect seen upon in vitro infection of CD4+ human T
cells by the HIV is cell-to-cell fusion that results in giant cell (or
syncytium) formation. Membrane fusion is required for infection by
cellfree virions and in syncytium formation. We report here that the
human T cell surface molecule, CD7, is important for the HIV-1 fusion
process. CD7 is a roughly 40-kDa glycoprotein member of the Ig supergene
family that is expressed early in the ontogeny of thymocytes and on the
majority of peripheral blood T cells, as well as on NK cells and a small
subpopulation of B cells. Anti-CD7 mAbs inhibited HIV-1-induced
cell-cell fusion and prevented cellfree infection of SupT1 cells. The
antisyncytial activity of the CD7 Abs is not because of cross-reactivity
with CD4 or with viral proteins. Epitope mapping revealed at least two
regions of the molecule that are important for preventing membrane
fusion. Cells rendered CD7- are poorly infectable by cellfree virus.
Additionally, cells rendered CD7- are more easily inhibited from fusing
in syncytium formation assays. The collective results support a central
role for human CD7 in the process of HIV infection.
DE Antibodies, Monoclonal/IMMUNOLOGY Antigenic Determinants Antigens,
CD/IMMUNOLOGY/*PHYSIOLOGY Antigens, CD4/IMMUNOLOGY Antigens,
Differentiation, T-Lymphocyte/IMMUNOLOGY/*PHYSIOLOGY Cell Fusion Cell
Line Cross Reactions Cytopathogenic Effect, Viral Human
HIV-1/*PATHOGENICITY Support, Non-U.S. Gov't Support, U.S. Gov't,
P.H.S. T-Lymphocytes/IMMUNOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).